Downstream processing of Cry4AaCter-induced inclusion bodies containing insect-derived antimicrobial peptides produced in Escherichia coli
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منابع مشابه
FTIR Investigation of Secondary Structure of Reteplase Inclusion Bodies Produced in Escherichia coli in Terms of Urea Concentration
Recent studies suggest that reducing the induction temperature would improve the quality of some recombinant inclusion bodies (IB) by providing a native-like secondary structure and leading to an improvement in protein recovery. This study focused on optimizing the solubilization condition of Reteplase, a recombinant protein with 9 disulfide bonds. The influence of lowering induction temperatur...
متن کاملFTIR Investigation of Secondary Structure of Reteplase Inclusion Bodies Produced in Escherichia coli in Terms of Urea Concentration
Recent studies suggest that reducing the induction temperature would improve the quality of some recombinant inclusion bodies (IB) by providing a native-like secondary structure and leading to an improvement in protein recovery. This study focused on optimizing the solubilization condition of Reteplase, a recombinant protein with 9 disulfide bonds. The influence of lowering induction temperatur...
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Overexpression of cloned or synthetic genes in Escherichia coli often results in the formation of insoluble protein inclusion bodies. Within the last decade, specific methods and strategies have been developed for preparing active recombinant proteins from these inclusion bodies. Usually, the inclusion bodies can be separated easily from other cell components by centrifugation, solubilized by d...
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24 Buchanan, S. K., Smith, B. S., Venkatramani. L., Xia, D., Esser, L.. Palnitkar, M.. Chakraborty, R, van der Helm, D. and Deisenhofer, J. ( I 999) Nat. Struct. Biol. 6, 5 6 6 3 Jalal, M. A. F. and van der Helm, D. (1989) FEBS Lett. 243, 366370 Sambrook 1.. Fritsch, E. F. and Maniatis, T. ( I 989) in Molecular Cloning: a Laboratory Manual, 2nd edn., Cold Spring Harbor Press, Cold Spring Harbor...
متن کاملOptimizing Primary Recovery and Refolding of Human Interferon-b from Escherichia coli Inclusion Bodies
Background: The refolding of proteins from inclusion bodies is affected by several factors, including solubilization of inclusion bodies by denaturants, removal of the denaturant, and assistance of refolding by small molecule additives. Objectives: The purpose of this study was optimization of recombinant human interferon-b purification in order to achieve higher efficiency, yield, and a produ...
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ژورنال
عنوان ژورنال: Protein Expression and Purification
سال: 2019
ISSN: 1046-5928
DOI: 10.1016/j.pep.2018.12.002